Bottiroli G, Docchio F, Freitas I, Ramponi R, Sacchi C A
Chem Biol Interact. 1984 Jul;50(2):153-7. doi: 10.1016/0009-2797(84)90092-9.
This work reports on studies of hematoporphyrin-derivative (HpD) behaviour in culture medium. Absorption, excitation and emission spectra, together with time-resolved fluorescence measurements, were performed. In previous works, similar studies had been carried out on HpD in saline and in lymphocytes: a new porphyrin species (NPS) and the environmental conditions for its formation in saline were studied. A fluorescent emission similar to that presented by the NPS is reported to be more likely in tumor rather than in normal HpD-treated cells, it was also found in greater amounts in lymphocytes in the pre-replicative phase, as compared with quiescent ones. The higher NPS content in stimulated rather than in quiescent lymphocytes may be due either to a differential uptake, as compared with other HpD components, or to a differential formation rate in cells, because of different microenvironmental conditions. To distinguish between these two main assumptions, the formation of NPS in culture medium was studied. The process was very slow: no NPS appeared within the first 40 h. The incubation time of lymphocytes in culture medium added with HpD in the experiments performed was only 1 h and therefore a differential formation rate of NPS may explain the higher content found in stimulated lymphocytes.
本研究报告了血卟啉衍生物（HpD）在培养基中的行为研究。进行了吸收光谱、激发光谱和发射光谱以及时间分辨荧光测量。在之前的研究中，已经对HpD在盐水和淋巴细胞中的情况进行了类似研究：研究了一种新的卟啉物种（NPS）及其在盐水中形成的环境条件。据报道，与正常HpD处理的细胞相比，肿瘤细胞中更可能出现与NPS相似的荧光发射，并且与静止淋巴细胞相比，在复制前期的淋巴细胞中也发现了更多的这种荧光发射。与静止淋巴细胞相比，受刺激淋巴细胞中较高的NPS含量可能是由于与其他HpD成分相比的差异摄取，或者是由于不同的微环境条件导致细胞中不同的形成速率。为了区分这两个主要假设，研究了培养基中NPS的形成。这个过程非常缓慢：在最初的40小时内没有出现NPS。在进行的实验中，淋巴细胞在添加了HpD的培养基中的孵育时间仅为1小时，因此NPS的不同形成速率可能解释了在受刺激淋巴细胞中发现的较高含量。
