Pringle M J, Sanadi D R
Membr Biochem. 1984;5(3):225-41. doi: 10.3109/09687688409150280.
Beef heart mitochondrial H+-ATPase (F1-F0) vesicles were prepared by lysolecithin extraction of ETPH. ATP-driven membrane potential was monitored indirectly by following absorbance changes of the potential-sensitive dye oxonol VI. The steady-state potential was discharged by oligomycin and/or Cd2+ (a dithiol reagent). At 13 degrees C, the agents appeared to act synergistically; at 24 degrees C the data were equivocal. When Cd2+ was added before energization, the membrane potential was markedly attenuated. Both effects of Cd2+ were inhibited by dithiothreitol. The activation energy for oligomycin-sensitive ATPase exhibited a discontinuity at 16 degrees C. However, the temperature dependence of the rate of potential discharge by oligomycin showed no such discontinuity. The results are discussed in terms of the involvement of thiol groups in proton translocation and the thermotropic behavior of the membrane vesicles.
通过溶血卵磷脂提取电子传递磷酸化颗粒（ETPH）制备牛肉心线粒体H⁺-ATP酶（F1-F0）囊泡。通过跟踪电位敏感染料氧杂萘邻酮VI的吸光度变化间接监测ATP驱动的膜电位。稳态电位通过寡霉素和/或Cd²⁺（一种二硫醇试剂）释放。在13℃时，这些试剂似乎具有协同作用；在24℃时，数据不明确。当在通电前加入Cd²⁺时，膜电位明显减弱。Cd²⁺的两种作用均被二硫苏糖醇抑制。寡霉素敏感ATP酶的活化能在16℃时出现不连续性。然而，寡霉素导致电位释放速率的温度依赖性未显示出这种不连续性。根据巯基在质子转运中的作用以及膜囊泡的热致行为对结果进行了讨论。
