Charnas R L, Goldberg I H
Biochem Biophys Res Commun. 1984 Jul 31;122(2):642-8. doi: 10.1016/s0006-291x(84)80081-9.
The oxidative reaction of polydeoxyadenylic-deoxythymidylic acid [poly(dA-dT)] with neocarzinostatin that produces 5'-thymidine aldehyde esterified to the 5'-end of strand breaks proceeds with hydrogen abstraction. The abstracted hydrogen is covalently bound to the non-protein component of neocarzinostatin; only a small amount (5%) is washed out into solvent. These data rule out a peroxyl radical as the primary DNA damaging species involved in the production of the 5'-aldehyde group. In contrast to earlier reports, it is demonstrated that alpha-tocopherol is not an inhibitor of the reaction.
聚脱氧腺苷酸-脱氧胸苷酸[聚（dA-dT）]与新制癌菌素的氧化反应会产生5'-胸腺嘧啶醛，该醛与链断裂的5'-末端酯化，此反应通过氢提取进行。提取的氢共价结合到新制癌菌素的非蛋白质成分上；只有少量（5%）被洗脱到溶剂中。这些数据排除了过氧自由基是参与5'-醛基产生的主要DNA损伤物种。与早期报告相反，已证明α-生育酚不是该反应的抑制剂。
