Michalopoulos G, Houck K A, Dolan M L, Leutteke N C
Cancer Res. 1984 Oct;44(10):4414-9.
Serum proteins from hepatectomized or control rats were separated by gel permeation chromatography and assayed for stimulation of hepatocyte proliferation in primary cultures of hepatocytes. Two peaks of activity were seen in the areas of large (greater than 120,000) and small (less than 3,000) molecular weight. These activities are different from insulin, epidermal growth factor, or vasopressin and are empirically termed hepatopoietin A and B, respectively. The two activities interact in a synergistic manner to stimulate hepatocyte proliferation at rates comparable to that of the whole serum.
通过凝胶渗透色谱法分离肝切除大鼠或对照大鼠的血清蛋白，并在原代肝细胞培养物中测定其对肝细胞增殖的刺激作用。在大分子（大于120,000）和小分子（小于3,000）分子量区域观察到两个活性峰。这些活性不同于胰岛素、表皮生长因子或血管加压素，根据经验分别称为肝细胞生成素A和B。这两种活性以协同方式相互作用，以与全血清相当的速率刺激肝细胞增殖。
