Rohloff D, Laiblin C, Heidrich S
Berl Munch Tierarztl Wochenschr. 1978 Jan 15;91(2):31-3.
The sperm-bearing fractions of 163 ejaculates collected from 6 Beagles aged two years were used to test the cryoprotective effect of DMSO and glycerol in deep freezing investigations. It could be shown that the cryoprotective capacity of glycerol was better than the one of DMSO. Glycerol should rather be added to the sperms at + 5 degrees C than at + 25 degrees C. The best preservation results were achieved by a combined addition of DMSO and glycerol under the following conditions: time of cooling down to +5 degrees C of the extended sperm fractions 50 minutes, final concentration of DMSO 1% (max.), final concentration of glycerol 8% (max.), glycerol equilibration time 30 minutes.
从6只两岁的比格犬采集的163份精液的含精子部分用于测试二甲基亚砜（DMSO）和甘油在深度冷冻研究中的冷冻保护作用。结果表明，甘油的冷冻保护能力优于DMSO。甘油应在+5℃而非+25℃时添加到精子中。在以下条件下联合添加DMSO和甘油可获得最佳保存效果：精液稀释液冷却至+5℃的时间为50分钟，DMSO最终浓度为1%（最大值），甘油最终浓度为8%（最大值），甘油平衡时间为30分钟。
