Welliver R C, Kaul T N, Sun M, Ogra P L
J Immunol. 1984 Oct;133(4):1925-30.
The relationship of suppressor cell numbers and function to virus-specific IgE response was determined in 72 infants with respiratory syncytial virus (RSV) infection. Monoclonal antibodies to membrane antigens were used to enumerate OKT4 and OKT8 antigen-positive cells, and suppressor cell function as quantitated by the degree of suppression of lymphocyte mitogenesis induced by incubation of lymphocyte cultures with histamine. Patients with bronchiolitis had fewer OKT8-positive cells during convalescence than patients with other forms of illness due to RSV (p less than 0.05). An inverse correlation of OKT8-positive cell numbers and peak IgE titers was observed (p less than 0.01). Histamine-induced suppression was also reduced in patients with bronchiolitis (p less than 0.05). In patients with repeated infection, improved histamine-induced suppression was associated with reduced virus-specific IgE titers and the absence of wheezing. Defects in immunoregulation may underlie previously recognized immunologic and pharmacologic abnormalities in patients with bronchiolitis.
在72例呼吸道合胞病毒（RSV）感染的婴儿中，确定了抑制细胞数量和功能与病毒特异性IgE反应的关系。使用针对膜抗原的单克隆抗体来计数OKT4和OKT8抗原阳性细胞，并通过将淋巴细胞培养物与组胺一起孵育诱导的淋巴细胞有丝分裂抑制程度来定量抑制细胞功能。毛细支气管炎患者在恢复期的OKT8阳性细胞比因RSV感染导致的其他疾病形式的患者少（p<0.05）。观察到OKT8阳性细胞数量与IgE峰值滴度呈负相关（p<0.01）。组胺诱导的抑制在毛细支气管炎患者中也降低（p<0.05）。在反复感染的患者中，组胺诱导的抑制改善与病毒特异性IgE滴度降低和无喘息相关。免疫调节缺陷可能是毛细支气管炎患者先前公认的免疫和药理学异常的基础。
