Argyris B F
Transplantation. 1984 Sep;38(3):247-51. doi: 10.1097/00007890-198409000-00010.
Suppressor cell activity in the spleen of 4-5-day-old mice is high and can be assayed in mixed lymphocyte cultures. The suppressor cells inhibit the proliferation of adult mouse spleen cells responding in vitro to an allogeneic stimulus. This suppressor cell activity can be increased by injecting the mice, on the day of birth, with 10(7) allogeneic spleen cells from adult mice. The suppressor cell activity in alloantigen stimulated neonatal mice is sensitive to treatment with anti-Thy-1.2 and complement. The increased neonatal suppressor cell activity after alloantigen stimulation has been demonstrated in a number of strain combinations. Preirradiation of the allogeneic adult donor spleen cells with 2,000 rads eliminates the ability of the spleen cells to increase neonatal suppressor cell activity. Nylon-wool-nonadherent (T-cell-enriched) spleen cells are most effective, and treatment of these cells with anti-Thy-1.2 and complement eliminates their activity. By using the appropriate strain combinations it was determined that the increase in neonatal suppressor cell activity is primarily due to a graft-versus-host reaction.
4至5日龄小鼠脾脏中的抑制细胞活性较高，且可在混合淋巴细胞培养物中进行检测。这些抑制细胞会抑制成年小鼠脾细胞在体外对同种异体刺激产生的增殖反应。通过在出生当天给小鼠注射10⁷个成年小鼠的同种异体脾细胞，这种抑制细胞活性能够增强。同种异体抗原刺激的新生小鼠中的抑制细胞活性对用抗Thy-1.2和补体进行的处理敏感。在许多品系组合中都证实了同种异体抗原刺激后新生小鼠抑制细胞活性的增强。用2000拉德对同种异体成年供体脾细胞进行预照射会消除脾细胞增强新生小鼠抑制细胞活性的能力。尼龙毛非黏附（富含T细胞）的脾细胞最为有效，用抗Thy-1.2和补体处理这些细胞会消除其活性。通过使用合适的品系组合确定，新生小鼠抑制细胞活性的增强主要是由于移植物抗宿主反应。
