酵母可溶性磷酸果糖激酶调节亚基中的突变。

Mutations in the regulatory subunit of soluble phosphofructokinase from yeast.

作者信息

Nadkarni M, Parmar L, Lobo Z, Maitra P K

出版信息

FEBS Lett. 1984 Oct 1;175(2):294-8. doi: 10.1016/0014-5793(84)80754-1.

Abstract

Mutant alleles of the gene PFK2 have been obtained that alter the sensitivity to ATP inhibition of the soluble yeast phosphofructokinase. One of the alleles makes the enzyme sensitive to micromolar concentrations of ATP. Intragenic revertants of PFK2 mutants confirm that the PFK2 gene determines not only the regulatory properties of the soluble enzyme but also the catalytic activity of particulate phosphofructokinase.

摘要

已获得基因PFK2的突变等位基因，这些突变等位基因改变了可溶性酵母磷酸果糖激酶对ATP抑制的敏感性。其中一个等位基因使该酶对微摩尔浓度的ATP敏感。PFK2突变体的基因内回复突变证实，PFK2基因不仅决定可溶性酶的调节特性，还决定颗粒状磷酸果糖激酶的催化活性。