针对21号染色体编码的细胞表面成分的抗体可阻断人α干扰素而非γ干扰素与人细胞的结合。

Antibodies to chromosome 21 coded cell surface components block binding of human alpha interferon but not gamma interferon to human cells.

作者信息

Shulman L M, Kamarck M E, Slate D L, Ruddle F H, Branca A W, Baglioni C, Maxwell B L, Gutterman J, Anderson P, Nagler C, Vilcek J

机构信息

Department of Biology, Yale University, New Haven, Connecticut 06520, USA.

出版信息

Virology. 1984 Sep;137(2):422-7. doi: 10.1016/0042-6822(84)90235-6.

DOI:10.1016/0042-6822(84)90235-6

PMID:6237488

Abstract

Antisera raised against a human X mouse hybrid cell line containing human chromosome 21 as its only human chromosome, block induction of an antiviral state by human alpha interferon (IFN-alpha), block induction of (2'-5')oligoisoadenylate synthetase [2'-5')A synthetase), and block binding of 125I-labeled and 35S-labeled recombinant, human IFN-alpha A, but not 125I-labeled IFN-gamma, to cell surface receptors. The data presented clearly demonstrate that the cell surface receptors for IFN-alpha and IFN-gamma are different, and provide independent evidence of the role of a chromosome 21 coded cell surface molecule in the pathway to the generation of the antiviral state.

摘要

针对一种人-小鼠杂交细胞系制备的抗血清，该杂交细胞系仅含人类21号染色体作为其唯一的人类染色体，可阻断人α干扰素（IFN-α）诱导抗病毒状态，阻断（2'-5'）寡聚异腺苷酸合成酶[（2'-5'）A合成酶]的诱导，并阻断125I标记和35S标记的重组人IFN-αA与细胞表面受体的结合，但不阻断125I标记的IFN-γ与细胞表面受体的结合。所呈现的数据清楚地表明，IFN-α和IFN-γ的细胞表面受体是不同的，并为21号染色体编码的细胞表面分子在抗病毒状态产生途径中的作用提供了独立证据。