Gulya K, Kása P
Acta Biol Hung. 1983;34(4):395-405.
The distribution of muscarinic cholinergic receptors, choline acetyl-transferase and acetylcholinesterase activities were measured in subcellular fractions of the rat striatum on the 5th and 15th days postnatally and in adulthood. The receptor density in the striatum of 5 and 15-day-old rats was 15%, respectively, of the adult value. Similar increases of the receptors could be detected in the synaptosomal and microsomal fractions in the postnatal life of rat. The activity of choline acetyltransferase on the same days was 15% and 28%. In the subcellular fractions, the enzyme activity was the highest in the microsomal fraction on both the 5th and 15th days postnatally. The activity of acetylcholinesterase in the homogenate was 6% of the adult value in the 5-day-old rat striatum, while in the synaptosomal fraction it was 11% and 47% of the adult value on the 5th and 15th days, respectively. Our results show that the development of the muscarinic cholinergic receptors precedes that of the two cholinergic enzymes in both 5 and 15-day-old rat striatum. This may suggest an early perikaryonal synthesis and the fast translocation of receptors to the axon terminals during ontogenetic development.
在出生后第5天、第15天以及成年期，对大鼠纹状体的亚细胞组分中的毒蕈碱型胆碱能受体分布、胆碱乙酰转移酶和乙酰胆碱酯酶活性进行了测定。5日龄和15日龄大鼠纹状体中的受体密度分别为成年大鼠的15%。在大鼠出生后的生命过程中，在突触体和微粒体组分中也能检测到类似的受体增加情况。同一天胆碱乙酰转移酶的活性分别为15%和28%。在亚细胞组分中，出生后第5天和第15天，微粒体组分中的酶活性最高。5日龄大鼠纹状体匀浆中乙酰胆碱酯酶的活性为成年大鼠的6%，而在突触体组分中，第5天和第15天分别为成年大鼠的11%和47%。我们的结果表明，在5日龄和15日龄大鼠纹状体中，毒蕈碱型胆碱能受体的发育先于两种胆碱能酶。这可能表明在个体发育过程中，早期胞体合成且受体快速转运至轴突终末。
