Moorman A F, de Boer P A, Linders M T, Charles R
Cell Differ. 1984 Jun;14(2):113-23. doi: 10.1016/0045-6039(84)90036-8.
The presumptive histone H5 of Xenopus laevis has been characterized by SDS and acid-urea-Triton polyacrylamide gel electrophoresis and compared with chicken histone H5. Chicken H5 has a lower electrophoretic mobility compared to that of Xenopus H5 in both gel systems. It is shown, using a polyclonal antiserum against chicken H5, that the Xenopus histone H5 is immunologically related to chicken histone H5. Monoclonal antibodies have been prepared to the Xenopus histone types H5 and H1A, that do not cross-react, as determined by their reactivity in an enzyme linked immunosorbent assay and by their ability to react with either H1A or H5 in an immunochemical test on total erythrocyte histones that are transferred to nitrocellulose after fractionation by SDS- or acid-urea polyacrylamide gel electrophoresis. As all nuclei of erythrocytes from adult Xenopus laevis can be shown to contain histone H1A and H5, these monoclonal antibodies can be used to further delineate the role of H5 in tissue differentiation.
非洲爪蟾的假定组蛋白H5已通过SDS以及酸性尿素-曲拉通聚丙烯酰胺凝胶电泳进行了表征，并与鸡组蛋白H5进行了比较。在两种凝胶系统中，鸡H5的电泳迁移率均低于非洲爪蟾H5。使用针对鸡H5的多克隆抗血清表明，非洲爪蟾组蛋白H5与鸡组蛋白H5在免疫上相关。已制备出针对非洲爪蟾组蛋白类型H5和H1A的单克隆抗体，通过酶联免疫吸附测定中的反应性以及在SDS或酸性尿素聚丙烯酰胺凝胶电泳分级分离后转移至硝酸纤维素膜上的全红细胞组蛋白的免疫化学测试中与H1A或H5反应的能力确定，它们不会发生交叉反应。由于成年非洲爪蟾红细胞的所有细胞核均显示含有组蛋白H1A和H5，因此这些单克隆抗体可用于进一步阐明H5在组织分化中的作用。
