Shas S, Pearson D J
Biochim Biophys Acta. 1978 Feb 13;539(1):12-8. doi: 10.1016/0304-4165(78)90116-2.
Phenobarbitone administered in drinking water (0.5 g/l) or by daily intraperitoneal injection (100 mg/kg body weight) consistently caused an elevation of hepatic NADP-linked malic enzyme in rats maintained on a pellet diet. Three to four days appeared to be required for maximum response. The effect was also observed in animals maintained on a protein rich diet, in which the basic hepatic malic enzyme activity was low, but not in animals maintained on a sucrose rich diet, in which the basic enzyme activity was almost twice normal. Methyl cholanthrene, administered by daily intraperitoneal injection (40 mg/kg body weight), resulted in elevated hepatic levels not only of malic enzyme but also of the pentose phosphate pathway dehydrogenases. The timing of the "starve-refeed" response of the hepatic NADP-linked dehydrogenases in phenobarbitone-treated rats was similar to that in controls, and similar maximum enzyme activities were reached. The role of cytoplasmic NADP-linked dehydrogenases in the provision of reducing equivalent is discussed, particularly in relation to hepatic microsomal drug metabolism.
给以颗粒饲料喂养的大鼠饮用含苯巴比妥（0.5 g/l）的水或每日腹腔注射（100 mg/kg体重），均会持续导致肝脏中与NADP相关的苹果酸酶升高。达到最大反应似乎需要三到四天。在以富含蛋白质的饲料喂养的动物中也观察到了这种效应，这些动物肝脏中的基础苹果酸酶活性较低，但在以富含蔗糖的饲料喂养的动物中未观察到，后者的基础酶活性几乎是正常水平的两倍。每日腹腔注射（40 mg/kg体重）甲基胆蒽，不仅会导致肝脏中苹果酸酶水平升高，还会导致磷酸戊糖途径脱氢酶水平升高。苯巴比妥处理的大鼠肝脏中与NADP相关的脱氢酶的“饥饿-再喂养”反应时间与对照组相似，并且达到了相似的最大酶活性。本文讨论了细胞质中与NADP相关的脱氢酶在提供还原当量方面的作用，特别是与肝脏微粒体药物代谢的关系。
