Kudriavtseva G V, Tsarenko E P
Biokhimiia. 1980 Apr;45(4):594-600.
The effects of prostaglandin F2 alpha (0,004--0,4 mg per 100 g) on the activity of NADP-dependent dehydrogenases from rat liver and uterus were studied. After 4 hrs following F2 alpha injection the activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase was found to increase 4,3- and 1,2-fold in the liver and 5- and 1,6-fold in the uterus, respectively. The content of glucose in the blood remained practically unchanged. No correlation between the amount of F2 alpha and the activities of the pentose phosphate pathway dehydrogenases was observed. The effect of F2 alpha on the dehydrogenases from rat uterus (but not liver) depended on the phase of the sexual cycle and reached its maximum in the period of the secretory activity (estrus). The administration of F2 alpha in combination with alpha- and beta-adrenergic blockers (phentolamine or propranolol) did not only enhance but also potentiated their stimulating effects on the activities of the dehydrogenases. F2 alpha shifted the ratio of reduced and oxidized forms of pyridine nucleotides in the liver, increasing it by 11,2 and 33,3% for NADPH/NADP+ and NADH/NADP+, respectively. The NADP-dependent isocitrate, malate and glutamate dehydrogenases from rat liver and uterus as well as the non-oxidizing enzymes of the pentose phosphate pathway of carbohydrate metabolism were insensitive to F2 alpha.
研究了前列腺素F2α（每100克0.004 - 0.4毫克）对大鼠肝脏和子宫中依赖烟酰胺腺嘌呤二核苷酸磷酸（NADP）的脱氢酶活性的影响。注射F2α后4小时，发现肝脏中葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶的活性分别增加了4.3倍和1.2倍，子宫中分别增加了5倍和1.6倍。血液中的葡萄糖含量实际上保持不变。未观察到F2α的量与磷酸戊糖途径脱氢酶活性之间的相关性。F2α对大鼠子宫（而非肝脏）脱氢酶的影响取决于性周期阶段，在分泌活动期（发情期）达到最大。联合给予F2α与α和β肾上腺素能阻滞剂（酚妥拉明或普萘洛尔）不仅增强了它们对脱氢酶活性的刺激作用，而且使其作用增强。F2α改变了肝脏中吡啶核苷酸还原型和氧化型的比例，使烟酰胺腺嘌呤二核苷酸磷酸（NADPH）/烟酰胺腺嘌呤二核苷酸磷酸（NADP +）和烟酰胺腺嘌呤二核苷酸（NADH）/烟酰胺腺嘌呤二核苷酸磷酸（NADP +）分别增加了11.2%和33.3%。大鼠肝脏和子宫中依赖NADP的异柠檬酸脱氢酶、苹果酸脱氢酶和谷氨酸脱氢酶以及碳水化合物代谢磷酸戊糖途径的非氧化酶对F2α不敏感。
