Tishchenko V M
Biofizika. 1978 Jan-Feb;23(1):153-5.
The molecular weight of butyrylcholinesterase from horse blood serum was determined using the method of analytical ultracentrifugation. The molecular weight of the enzyme subunit was also determined by its mobility in gel containing sodium dodecyl sulphate. The number of active sites was determined by titrating butyrylcholinesterase with a specific inhibitor, namely di-isopropyl phosphorofluoridate. A conclusion is made that butyrylcholinesterase is a tetramer with one active site per unit.
采用分析超速离心法测定了马血清中丁酰胆碱酯酶的分子量。还通过其在含十二烷基硫酸钠的凝胶中的迁移率测定了酶亚基的分子量。通过用一种特异性抑制剂（即二异丙基氟磷酸酯）滴定丁酰胆碱酯酶来确定活性位点的数量。得出的结论是，丁酰胆碱酯酶是一种四聚体，每个亚基有一个活性位点。
