Rafestin-Oblin M E, Claire M, Lombes M, Michaud A, Corvol P
J Steroid Biochem. 1984 Oct;21(4):465-70. doi: 10.1016/0022-4731(84)90313-3.
The numbers of type I and type II aldosterone receptors in the kidney cytosol of adrenalectomized rats were estimated after animals were treated with various steroids, or fed with high or low potassium diets. Oestradiol and 5 beta-pregnane-3,20 dione, which exhibited no affinity for aldosterone receptors, did not modify the levels of type I or type II receptors. Cortisol, corticosterone, progesterone and spirolactones, which all competed with aldosterone for both types of receptor, reduced the number of type I sites, as does aldosterone itself. Steroid treatment has no appreciable effect on type II receptors. We conclude that type I receptors are modulated by steroids able to bind to aldosterone receptors and that steroid-receptor interaction is an essential step in the receptor modulation process. The effects of potassium on aldosterone receptor modulation were tested in adrenalectomized rats on hypo- or hyperkalaemic diets. No change in receptor levels was observed in the rats on a low potassium diet, but the number of type I receptors increased in animals on a high potassium diet. However, the effects of potassium on receptor modulation were of lesser magnitude than those of aldosterone agonists and antagonists.
在对去肾上腺大鼠用各种类固醇进行处理，或给予高钾或低钾饮食后，对其肾胞质溶胶中I型和II型醛固酮受体的数量进行了估计。对醛固酮受体无亲和力的雌二醇和5β-孕烷-3,20-二酮，并未改变I型或II型受体的水平。皮质醇、皮质酮、孕酮和螺内酯，它们均与醛固酮竞争这两种类型的受体，与醛固酮本身一样，会减少I型受体位点的数量。类固醇处理对II型受体无明显影响。我们得出结论，I型受体受能够与醛固酮受体结合的类固醇调节，且类固醇-受体相互作用是受体调节过程中的一个关键步骤。在低钾或高钾饮食的去肾上腺大鼠中测试了钾对醛固酮受体调节的影响。低钾饮食的大鼠中未观察到受体水平的变化，但高钾饮食的动物中I型受体的数量增加。然而，钾对受体调节的影响程度小于醛固酮激动剂和拮抗剂。
