Pope R M, McChesney L, Talal N, Fischbach M
Arthritis Rheum. 1984 Nov;27(11):1234-44. doi: 10.1002/art.1780271105.
In order to characterize the autologous mixed lymphocyte response (AMLR) in patients with rheumatoid arthritis (RA) and to define the relationship with disease activity, peripheral blood T lymphocytes were stimulated with either a B lymphocyte-enriched (B cells) or a macrophage-enriched (macrophages) population. A significant reduction (P less than 0.01 to P less than 0.001) of T cell proliferation stimulated both by B cells and macrophages was observed in patients with active disease. The B lymphocytes were significantly less stimulatory (P less than 0.02 to P less than 0.001) than macrophages in the patients compared with the controls. In the normal controls, macrophages in higher concentrations were capable of suppressing the B lymphocyte-stimulated AMLR, but macrophages from patients with RA were not excessively suppressive. A significant association (P less than 0.02) was observed between disease activity and the AMLR. Using the B-enriched population, the AMLR proliferative response was significantly associated (P less than 0.001) with the production of interleukin-2. Defects in proliferation could only be partially restored by the addition of interleukin-2. These data indicate that the defective AMLR observed in patients with RA is related to disease activity and is associated with altered cellular interactions among T lymphocytes, macrophages, and the B lymphocyte-enriched population.
为了描述类风湿关节炎（RA）患者的自体混合淋巴细胞反应（AMLR）并确定其与疾病活动的关系，用富含B淋巴细胞（B细胞）或富含巨噬细胞（巨噬细胞）的群体刺激外周血T淋巴细胞。在活动期疾病患者中，观察到B细胞和巨噬细胞刺激的T细胞增殖均显著降低（P小于0.01至P小于0.001）。与对照组相比，患者中的B淋巴细胞刺激作用明显小于巨噬细胞（P小于0.02至P小于0.001）。在正常对照组中，较高浓度的巨噬细胞能够抑制B淋巴细胞刺激的AMLR，但类风湿关节炎患者的巨噬细胞没有过度抑制作用。观察到疾病活动与AMLR之间存在显著关联（P小于0.02）。使用富含B细胞的群体，AMLR增殖反应与白细胞介素-2的产生显著相关（P小于0.001）。添加白细胞介素-2只能部分恢复增殖缺陷。这些数据表明，类风湿关节炎患者中观察到的AMLR缺陷与疾病活动有关，并且与T淋巴细胞、巨噬细胞和富含B淋巴细胞的群体之间细胞相互作用的改变有关。
