Kodaira K, Taketo A
Biochim Biophys Acta. 1984 Nov 22;783(2):171-8. doi: 10.1016/0167-4781(84)90010-1.
Various mutants were isolated from a microvirid (isometric single-stranded DNA) phage alpha 3, by mutagenesis with hydroxylamine or nitrous acid. They were divided into eight complementation groups, and mainly by genetic crosses the gene alignment was determined as -A-B-C'-D-J'-F-G-H-. Except for groups C' and J', each defective gene product was clearly discerned in electropherograms of proteins extracted from the phage-infected suppressor-negative (Su-) Escherichia coli. Only gene A mutants abolished synthesis of the progeny replicative-form DNA (RF), whereas mutants belonging to groups B, C', D, E, F and J' affected RF replication at late stage, as well as synthesis of the single-stranded DNA (SS). Additional properties of several mutants are also discussed.
通过用羟胺或亚硝酸诱变，从一种微小病毒（等轴单链DNA）噬菌体α3中分离出各种突变体。它们被分为八个互补群，主要通过遗传杂交确定基因排列为-A-B-C'-D-J'-F-G-H-。除了C'和J'组外，在从噬菌体感染的抑制因子阴性（Su-）大肠杆菌中提取的蛋白质的电泳图谱中可以清楚地识别出每个缺陷基因产物。只有A组突变体消除了子代复制型DNA（RF）的合成，而属于B、C'、D、E、F和J'组的突变体在后期影响RF复制以及单链DNA（SS）的合成。还讨论了几种突变体的其他特性。
