Arai K, Arai N, Shlomai J, Kornberg A
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3322-6. doi: 10.1073/pnas.77.6.3322.
Replication of the covalently closed duplex replicative form (RF) of phage phi X174 DNA has been achieved by coupling two known enzyme systems: (i) synthesis of viral strand circles (SS) from RF, and (ii) conversion of SS to nearly complete RF (RF II). In this coupled system, activated RF (gene A . RF II complex) was a more efficient template and generated as many as 10 RF II molecules per RF input, at a rate commensurate with SS synthesis. The 11 proteins required for the two component systems were all needed in the coupled RF duplication system; no new factors were required. Single-stranded DNA binding protein was needed for RF duplication at only 4% the level needed in its stoichiometric participation in SS synthesis. In addition to RF II, more complex replicative forms appeared late in the reaction, and their possible origin is discussed.
通过耦合两个已知的酶系统，实现了噬菌体φX174 DNA共价闭合双链复制形式（RF）的复制：（i）从RF合成病毒链环（SS），以及（ii）将SS转化为近乎完整的RF（RF II）。在这个耦合系统中，活化的RF（基因A·RF II复合物）是一种更有效的模板，每个输入的RF可产生多达10个RF II分子，其速率与SS合成相当。两个组成系统所需的11种蛋白质在耦合的RF复制系统中都是必需的；不需要新的因子。单链DNA结合蛋白在RF复制中的需求量仅为其化学计量参与SS合成所需水平的4%。除了RF II，反应后期还出现了更复杂的复制形式，并对其可能的起源进行了讨论。
