Kodaira K, Taketo A
Mol Gen Genet. 1984;195(3):541-3. doi: 10.1007/BF00341460.
To elucidate the in vivo function of the J gene of microvirid (isometric) phages, we isolated several strains carrying a double mutation in J and H genes from phage alpha3 and then constructed single mutants each having an amber codon in the J gene. The J mutants could not multiply in suppressor-less hosts and were deficient in single-stranded progeny DNA synthesis. Nucleotide sequences of the wild-type and mutant alpha# J genes were analyzed to determine the mutation sites. The amino acid sequence of the J gene was also deduced from the nucleotide sequence and compared with those of phiX174 and G4.
为阐明微小病毒（等轴）噬菌体J基因的体内功能，我们从噬菌体α3中分离出了几株在J基因和H基因中携带双突变的菌株，然后构建了J基因中各自带有一个琥珀密码子的单突变体。J突变体无法在无抑制因子的宿主中繁殖，并且在单链子代DNA合成方面存在缺陷。分析野生型和突变型α# J基因的核苷酸序列以确定突变位点。还从核苷酸序列推导了J基因的氨基酸序列，并与φX174和G4的氨基酸序列进行了比较。
