Bowen B C, Zimm B H
Biophys Chem. 1978 Jan;7(4):235-52. doi: 10.1016/0301-4622(78)85001-7.
The viscoelastic properties of T2 DNA solutions are used to determine the NaDNA molecular weight in four independent ways from the theory of the beads-springs model. The four molecular weights are 131.9, 132.7, 130.5, and 127.6 X 10(6). The average of these values, adjusted for the probable errors in viscoelasticity and concentration measurements, is (126 +/- 5) X 10(6). The four molecular weights are termed Mtaugamma11, Mtaueta, Mtaugamma, and MtauA; each is different in its sensitivity to molecular weight distribution. Their agreement suggests (1) that the theoretical equations relating each M to the corresponding measured properties are valid, (2) that T2 DNA behaves as a partially free-draining polymer chain, and (3) that our solutions were nearly homogeneous in DNA size. We show that serious errors can result if the viscoelastic properties are not extrapolated to their limits at zero shear rate, as well as at zero DNA concentration, before calculating molecular weight.
利用T2 DNA溶液的粘弹性，根据珠-弹簧模型理论，通过四种独立方法测定了NaDNA的分子量。这四个分子量分别为131.9、132.7、130.5和127.6×10⁶。根据粘弹性和浓度测量中的可能误差进行调整后，这些值的平均值为(126±5)×10⁶。这四个分子量分别称为Mtaugamma11、Mtaueta、Mtaugamma和MtauA；它们对分子量分布的敏感性各不相同。它们的一致性表明：(1) 将每个M与相应测量性质相关联的理论方程是有效的；(2) T2 DNA表现为部分自由排水的聚合物链；(3) 我们的溶液在DNA大小方面几乎是均匀的。我们表明，如果在计算分子量之前，不将粘弹性性质外推到零剪切速率以及零DNA浓度时的极限值，可能会导致严重误差。
