痤疮制剂中过氧化苯甲酰的液相色谱测定法。
Liquid chromatographic determination of benzoyl peroxide in acne preparations.
作者信息
Chou C K, Locke D C
出版信息
J Assoc Off Anal Chem. 1984 Sep-Oct;67(5):913-5.
A rapid, precise, and accurate liquid chromatographic (LC) method is described for the determination of benzoyl peroxide (BP) in acne preparations. BP is extracted from a water dispersion of the preparation with dichloromethane (DCM), and an aliquot is eluted from a C-18 reverse phase LC column with acetonitrile-0.10 M aqueous NaClO4. Selective and sensitive quantitation is accomplished with a reductive mode electrochemical detector. This detector is an order of magnitude more sensitive than a 240 nm UV absorption detector; the lower limit of detection is 2 ng for a 4 microL injection. The recovery of BP is 99.4% and the detector response is linear to at least 2 micrograms per 4 microL injection.
本文描述了一种快速、精确且准确的液相色谱（LC）方法，用于测定痤疮制剂中的过氧化苯甲酰（BP）。BP从制剂的水分散体中用二氯甲烷（DCM）萃取，取一份等分试样用乙腈 - 0.10 M 高氯酸钠水溶液从 C - 18 反相 LC 柱上洗脱。采用还原模式电化学检测器实现选择性和灵敏的定量分析。该检测器比 240 nm 紫外吸收检测器灵敏一个数量级；对于 4 μL 进样，检测下限为 2 ng。BP 的回收率为 99.4%，并且检测器响应对于每 4 μL 进样至少 2 μg 呈线性关系。
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