Zolotukhin A S, Drygin Iu F, Bogdanov A A
Bioorg Khim. 1984 Aug;10(8):1109-13.
The 32P-labelled A* protein has been isolated from E. coli cells infected by phage phi X174 in the presence of [32P]orthophosphate. The snake venom phosphodiesterase treatment of the [32P]peptides obtained by the pronase digestion of the protein has revealed a phosphodiester bond between the protein and a nucleotide material of A, G base composition. The hydrolysis of nucleotide-peptides with a mixture of concentrated HCl and CF3COOH has yielded 4'O-phosphotyrosine.
在[32P]正磷酸盐存在的情况下，从被噬菌体φX174感染的大肠杆菌细胞中分离出了32P标记的A*蛋白。用链霉蛋白酶消化该蛋白所得到的[32P]肽段经蛇毒磷酸二酯酶处理后，揭示了该蛋白与一种A、G碱基组成的核苷酸物质之间存在磷酸二酯键。用浓盐酸和三氟乙酸的混合物水解核苷酸肽段产生了4'-磷酸酪氨酸。
