Sato T, Akatsuka H, Kito K, Tokoro Y, Tauchi H, Kato K
Mech Ageing Dev. 1984 Nov;28(1):99-109. doi: 10.1016/0047-6374(84)90156-8.
Micromeasuring studies have been made on the senile changes of the surgically resected minor pectoral muscles in 200 Japanese females of various ages (26-80 years). The microscopic differentiation between type I (red) and type II (white) fibers were made by the histochemically demonstrated myosin-ATPase activity with frozen sections and also by the immunohistochemical demonstration of beta-enolase in the paraffin sections with indirect antibody method. The decrease with age in weight of the muscle was not markedly observed. Muscle fibers of type I were significantly decreased in number and increased in size after 60 years of age. However, size of muscle fibers of type II decreased gradually after 40 years of age. However, size of muscle fibers of type II decreased gradually after 40 years of age. Total volume of type I muscle fibers was not changed according to age, on the contrary, that of the type II fibers decreased significantly after 60 years of age.
对200名不同年龄（26 - 80岁）的日本女性手术切除的胸小肌进行了微观测量研究，以观察其衰老变化。通过冷冻切片上组织化学显示的肌球蛋白 - ATP酶活性以及石蜡切片上采用间接抗体法免疫组织化学显示β - 烯醇化酶，对I型（红色）和II型（白色）纤维进行微观区分。未明显观察到肌肉重量随年龄下降。60岁以后，I型肌纤维数量显著减少，尺寸增大。然而，II型肌纤维尺寸在40岁以后逐渐减小。I型肌纤维的总体积并未随年龄变化，相反，II型纤维的总体积在60岁以后显著下降。
