Shaw M T
Semin Oncol. 1976 Sep;3(3):219-28.
A combination of cytomorphological features and cytochemical staining reactions in the diagnoses of the different types of acute leukemia removes much of the subjective difficulty of diagnosis. The number of cytochemical stains used may be limited, and in our experience the PAS, combined NCE and ANE reactions, and either SB or Px staining will yield as much diagnostic information as needed in most cases. With regard to the prognostic implications, it would appear at the present time that the amount of ANE staining in the cytoplasm of blasts is inversely proportional to the chances of remission induction and length of survival in cases of acute leukemia. However, further studies must be carried out to verify this finding before using the information to compliment other prognostic variables, such as age of patient, previous therapy, cytokinetics, etc.
细胞形态学特征与细胞化学染色反应相结合用于不同类型急性白血病的诊断，消除了许多诊断上的主观困难。所用细胞化学染色的数量可能有限，根据我们的经验，过碘酸雪夫反应（PAS）、中性粒细胞碱性磷酸酶（NCE）和酸性磷酸酶（ANE）联合反应，以及苏丹黑（SB）或酸性磷酸酶（Px）染色，在大多数情况下能提供所需的诊断信息。关于预后意义，目前看来，急性白血病病例中原始细胞胞质内酸性磷酸酶（ANE）染色量与缓解诱导的可能性及生存期呈反比。然而，在利用该信息补充其他预后变量（如患者年龄、既往治疗、细胞动力学等）之前，必须进行进一步研究以验证这一发现。
