Leipe S, Mohrig W
Allerg Immunol (Leipz). 1984;30(4):230-6.
The use of Cd microcrystals together with a monolayer technique using small quantities of whole blood offers several advantages for routine studies of phagocytosis. The original method by Miler was especially minimized with regard to the necessary quantity of serum. Homologous serum was replaced by heterologous guinea-pig serum and the method was simplified by the omission of opsonization. A comparison of the stimulatory effect of inactivated and native serum as well as the inhibition of phagocytosis by trypan blue allows us to characterize Cd-phagocytosis as complement-dependent.
使用镉微晶结合采用少量全血的单层技术，为吞噬作用的常规研究提供了几个优点。米勒的原始方法在所需血清量方面得到了特别优化。同源血清被异源豚鼠血清所取代，并且通过省略调理作用简化了该方法。对灭活血清和天然血清的刺激作用以及锥虫蓝对吞噬作用的抑制作用进行比较，使我们能够将镉吞噬作用表征为补体依赖性。
