Bellnier D A, Lin C W
Prog Clin Biol Res. 1984;170:361-71.
This study was designed to determine the influence, if any, of intracellular porphyrin aggregation upon in vitro photosensitivity. HpD uptake conditions were manipulated in such a way as to yield three experimental groups, each containing equal levels of cellular porphyrins but having different porphyrin aggregation status. Aggregation status was determined through comparison of the spectral properties of intact cells. Subsequent exposure to red light resulted in a family of survival curves which generally corresponded to cellular HpD fluorescence yields and optical densities (indicators of aggregation status). These data suggest that porphyrin aggregation within the cell may be partially responsible for the observed differential photosensitivity following various uptake conditions.
本研究旨在确定细胞内卟啉聚集（若有的话）对体外光敏性的影响。通过操控血卟啉衍生物（HpD）的摄取条件，产生了三个实验组，每组细胞内卟啉水平相等，但卟啉聚集状态不同。通过比较完整细胞的光谱特性来确定聚集状态。随后用红光照射，得到了一系列存活曲线，这些曲线总体上与细胞内HpD荧光产率和光密度（聚集状态指标）相对应。这些数据表明，细胞内的卟啉聚集可能部分导致了在不同摄取条件下观察到的光敏性差异。
