Kinsey J H, Cortese D A, Moses H L, Ryan R J, Branum E L
Cancer Res. 1981 Dec;41(12 Pt 1):5020-6.
Tumor cell killing effect of hematoporphyrin derivative (HPD) and light was studied in culture to determine the dependence of this effect on treatment variables. Particular attention has been given to the spectral characteristics of the light and the absorption properties of hematoporphyrin. A human tumor cell line was treated using HPD and three broad bands of light ranging from the short- to the long-wavelength end of the visible spectrum. Cell killing was assessed by trypan blue exclusion. A transformed mouse embryo cell line was treated in a similar manner, and its reproductive efficiency was determined following treatment. Results of both studies are consistent with the hypothesis that the photocytotoxic action of HPD plus light is directly proportional to the number of light quanta absorbed by the HPD in each cell. For thin layers of cells, such as in situ carcinoma, it appears that short-wavelength radiation falling in the porphyrin Soret band around 400 nm may have from 12 to 30 times the killing power as does red light.
在细胞培养中研究了血卟啉衍生物（HPD）与光对肿瘤细胞的杀伤作用，以确定这种作用对治疗变量的依赖性。特别关注了光的光谱特性和血卟啉的吸收特性。使用HPD和从可见光谱的短波端到长波端的三个宽波段光对一种人类肿瘤细胞系进行处理。通过台盼蓝排斥法评估细胞杀伤情况。以类似方式处理一种转化的小鼠胚胎细胞系，并在处理后测定其繁殖效率。两项研究的结果均与以下假设一致：HPD加光的光细胞毒性作用与每个细胞中HPD吸收的光量子数成正比。对于薄层细胞，如原位癌，落在约400nm卟啉Soret带的短波长辐射的杀伤能力似乎是红光的12至30倍。
