Ortez R A, Sikes R W, Sperling H G
J Histochem Cytochem. 1980 Mar;28(3):263-70. doi: 10.1177/28.3.6243683.
The immunocytochemical procedure for cyclic GMP (cGMP) localization has been modified by the development of: 1) an anti-cGMP antibody depleted control serum and 2) a tissue preadsorption procedure that greatly reduces nonspecific fluorescence. cGMP remaining associated with tissue sections following prolonged exposure to aqueous buffer was determined, by extraction and measurement by radioimmunoassay, to be approximately 38% of the initial cGMP content. Application of this improved immunohistochemical procedure to goldfish retinas reveals the highest concentrations of cGMP to be associated with the cone inner segments and nuclei of the photoreceptors. This retinal distribution of cGMP is in good agreement with that independently determined by microdissection studies. The validity of this procedure is therefore strengthened and cautions to its application and interpretation are outlined.
环磷酸鸟苷（cGMP）定位的免疫细胞化学方法已因以下两点进展而得到改进：1）一种去除了抗cGMP抗体的对照血清；2）一种能大幅减少非特异性荧光的组织预吸附程序。通过提取并用放射免疫测定法测量，确定长时间暴露于水性缓冲液后仍与组织切片结合的cGMP约为初始cGMP含量的38%。将这种改进的免疫组织化学方法应用于金鱼视网膜，结果显示cGMP浓度最高的部位与光感受器的视锥细胞内节和细胞核相关。cGMP在视网膜中的这种分布与通过显微切割研究独立确定的结果高度一致。因此，该方法的有效性得到了加强，并概述了其应用和解释时的注意事项。
