Ortez R A, Tamayo A, Johnson C, Sperling H G
J Histochem Cytochem. 1983 Nov;31(11):1305-11. doi: 10.1177/31.11.6311894.
A procedure for the immunocytochemical localization of cGMP in the goldfish retina is described. The procedure is then used to evaluate the presence of cGMP in cone color receptors in retinas from dark-adapted and light stimulate fish. The results show that cone color receptors in dark-adapted retinas contain large amounts of immunocytochemically defined cGMP in the ellipsoid region, which are greatly reduced after light exposure.
本文描述了一种在金鱼视网膜中对环鸟苷酸(cGMP)进行免疫细胞化学定位的方法。然后使用该方法评估暗适应和光刺激鱼视网膜中视锥颜色感受器中环鸟苷酸的存在情况。结果表明,暗适应视网膜中的视锥颜色感受器在椭球体区域含有大量通过免疫细胞化学确定的环鸟苷酸,光照后其含量大幅减少。