禽传染性支气管炎病毒结构多肽：不同裂解条件的影响以及不同毒株和分离株的比较
Avian infectious bronchitis virus structural polypeptides: effect of different conditions of disruption and comparison of different strains and isolates.
作者信息
Collins M S, Alexander D J
出版信息
Arch Virol. 1980;63(3-4):239-51. doi: 10.1007/BF01315030.
Variations in the conditions used for disruption of purified virus involving differences in heat treatment and reducing agent concentration produced little affect on the polypeptide profiles of the Massachusetts 41 (M 41) strain of avian infectious bronchitis virus obtained by polyacrylamide gel electrophoresis. Comparisons of the structural polypeptides of 12 IBV isolates, consiting of M 41, six serologically related viruses and representatives of five other serotypes, showed that the viruses could be placed in three groups on the molecular weights of the major glycopolypeptides. These were 31,000 and 86,000 for M 41, the six related viruses and the serologically distinct SE 17; 27,000 and 89,000 for Iowa 97 and Holte and 27,000 and 91,000 for Connecticut and T strain.
用于纯化病毒裂解的条件变化，包括热处理和还原剂浓度的差异，对通过聚丙烯酰胺凝胶电泳获得的禽传染性支气管炎病毒马萨诸塞41（M 41）株的多肽图谱影响很小。对12种传染性支气管炎病毒（IBV）分离株的结构多肽进行比较，这些分离株包括M 41、6种血清学相关病毒以及其他5种血清型的代表毒株，结果表明，根据主要糖多肽的分子量，这些病毒可分为三组。M 41、6种相关病毒和血清学不同的SE 17的分子量分别为31,000和86,000；爱荷华97和霍尔特的分子量为27,000和89,000；康涅狄格和T株的分子量为27,000和91,000。
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