禽传染性支气管炎病毒分离表面突起的多肽组成

The polypeptide composition of isolated surface projections of avian infectious bronchitis virus.

作者信息

Collins M S, Alexander D J

出版信息

J Gen Virol. 1980 May;48(1):213-7. doi: 10.1099/0022-1317-48-1-213.

Abstract

Disruption of avian infectious bronchitis virus (IBV) particles with 4% Triton X-100 and 1.0 M-KCl and centrifugation through a sucrose gradient containing 0.1% Triton X-100 and 1.0 M-KCl enabled separation of the petal-shaped surface projections. By negative-contrast electron microscopy the separated projections appeared mainly as rosettes containing 3 to 12 projections radiating from a central core, although single projections and rosettes containing up to 16 projections were seen. SDS-PAGE of these preparations revealed two polypeptides of 86,000 and 66,000 mol. wt. The larger polypeptide was glycosylated.

摘要

用4% Triton X - 100和1.0 M - KCl破坏禽传染性支气管炎病毒（IBV）颗粒，并通过含有0.1% Triton X - 100和1.0 M - KCl的蔗糖梯度离心，能够分离出花瓣状表面突起。通过负染电子显微镜观察，分离出的突起主要呈现为玫瑰花结，其中包含3至12个从中央核心放射状伸出的突起，不过也观察到了单个突起以及含有多达16个突起的玫瑰花结。这些制剂的SDS - PAGE显示出分子量为86,000和66,000的两种多肽。较大的多肽是糖基化的。