禽传染性支气管炎病毒的多肽组成
The polypeptide composition of avian infectious bronchitis virus.
作者信息
Bingham R W
出版信息
Arch Virol. 1975;49(2-3):207-16. doi: 10.1007/BF01317539.
Abstract
Avian infectious bronchitis virus grown in ovo was purified by differential centrifugation and isopycnic sedimentation in density gradients. The purified virus was analysed by SDS polyacrylamide gel electrophoresis and found to comprise up to sixteen polypeptides, four of which were glycopeptides. Bromelain treatment of the particles removed three polypeptides and two glycopeptides.
摘要
通过密度梯度中的差速离心和等密度沉降对在鸡胚内生长的禽传染性支气管炎病毒进行纯化。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对纯化后的病毒进行分析，发现其包含多达16种多肽，其中4种为糖肽。用菠萝蛋白酶处理病毒颗粒可去除3种多肽和2种糖肽。
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