在源自诱导型BALB/K - 3T3细胞的病毒分离物中检测B嗜性内源性C型病毒。

Detection of B-tropic endogenous type-C virus in viral isolates originating from induced BALB/K-3T3 cells.

作者信息

Laprevotte I, Chuat J C, Pilon C

出版信息

Intervirology. 1980;12(6):316-28. doi: 10.1159/000149091.

Abstract

Isoelectric focusing (IEF) analysis of class I endogenous type-C virus induced by iododeoxyuridine treatment of BALB/K-3T3 cells revealed, in addition to the major variant of the p30 polypeptide, which has an isoelectric point of 6.1 (pI 6.1 isop30), a minor isop30 with a pI of 5.6. This value was also found for a prototype BALB/c B-tropic endogenous virus isolate. The pI 5.6 isop30 of the N-tropic isolate was amplified by long-term virus replication in B-type mouse cells, and comparative IEF and XC-assay data suggest that it may correspond to a B-tropic subpopulation which has not yet been detected in vitro in mouse cells of embryonic origin.

摘要

对经碘脱氧尿苷处理的BALB/K - 3T3细胞诱导产生的I类内源性C型病毒进行等电聚焦（IEF）分析发现，除了等电点为6.1的p30多肽主要变体（pI 6.1 isop30）外，还有一个pI为5.6的次要isop30。在一种原型BALB/c B嗜性内源性病毒分离株中也发现了这个值。通过在B型小鼠细胞中进行长期病毒复制，扩增出了N嗜性分离株的pI 5.6 isop30，比较IEF和XC检测数据表明，它可能对应于一种尚未在胚胎来源的小鼠细胞体外检测到的B嗜性子群体。