一类独特的可诱导鼠类C型病毒，可在兔SIRC细胞系中复制。
A distinct class of inducible murine type-C viruses that replicates in the rabbit SIRC cell line.
作者信息
Benveniste R E, Lieber M M, Todaro G J
出版信息
Proc Natl Acad Sci U S A. 1974 Mar;71(3):602-6. doi: 10.1073/pnas.71.3.602.
The existence of the selectively permissive rabbit cell line SIRC allows definition of a new class of endogenous murine type-C virus. Continuous clonal lines of transformed cells derived from the BALB/c mouse-embryo cell line BALB/3T3 contain at least two distinct classes of endogenous type-C viral genomes. Spontaneously released endogenous viruses grow well on the mouse cell line NIH/3T3 (N-tropic viruses) but not on the rabbit cell line SIRC. Type-C viruses induced by treatment with BrdU grow well on SIRC (S-tropic viruses) but not in NIH/3T3 or BALB/3T3. BrdU-treated AKR mouse-embryo cells also release an S-tropic virus. N-tropic and S-tropic viruses both have the mouse intraspecies gs-1 and viral RNA-directed DNA polymerase antigenic determinants. DNA.RNA hybridization techniques reveal that the two host-range classes of endogenous viruses are only partially related to each other. Cell transformation facilitates the spontaneous release of the N-tropic viruses; treatment with thymidine analogues induces the production of the S-tropic viruses. Thus, the two classes of viral genomes appear to be subject to different cellular controls.
选择性允许的兔细胞系SIRC的存在使得能够定义一类新的内源性鼠C型病毒。源自BALB/c小鼠胚胎细胞系BALB/3T3的转化细胞的连续克隆系含有至少两类不同的内源性C型病毒基因组。自发释放的内源性病毒在小鼠细胞系NIH/3T3（N嗜性病毒）上生长良好，但在兔细胞系SIRC上不能生长。用溴脱氧尿苷（BrdU）处理诱导产生的C型病毒在SIRC（S嗜性病毒）上生长良好，但在NIH/3T3或BALB/3T3中不能生长。用BrdU处理的AKR小鼠胚胎细胞也释放一种S嗜性病毒。N嗜性和S嗜性病毒都具有小鼠种内gs-1和病毒RNA指导的DNA聚合酶抗原决定簇。DNA-RNA杂交技术表明，这两类宿主范围的内源性病毒彼此仅部分相关。细胞转化促进了N嗜性病毒的自发释放；用胸苷类似物处理诱导产生S嗜性病毒。因此，这两类病毒基因组似乎受到不同的细胞控制。
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