构建含有前α1（I）型胶原蛋白DNA序列的重组细菌质粒。
Construction of a recombinant bacterial plasmid containing pro-alpha 1(I) collagen DNA sequences.
作者信息
Yamamoto T, Sobel M E, Adams S L, Avvedimento V E, DiLauro R, Pastan I, de Crombrugghe B, Showalter A, Pesciotta D, Fietzek P, Olsen B
出版信息
J Biol Chem. 1980 Mar 25;255(6):2612-5.
PMID:6244285
Abstract
A partially purified mRNA preparation enriched for chick collagen messenger RNA activity was used as template for the synthesis of double stranded cDNA. The cDNA was ligated into the HindIII site of the plasmid vector pBR322 and used to transform Escherichia coli x1776. One plasmid with an 800-base pair insert was shown to contain DNA sequences corresponding to Type I pro-alpha 1 collagen.
摘要
一种富含鸡胶原蛋白信使核糖核酸活性的部分纯化信使核糖核酸制剂被用作合成双链互补脱氧核糖核酸的模板。该互补脱氧核糖核酸被连接到质粒载体pBR322的HindIII位点，并用于转化大肠杆菌x1776。一个带有800个碱基对插入片段的质粒被证明含有与I型原α1胶原蛋白相对应的DNA序列。
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