Ishii S, Hatada E, Maekawa T, Imamoto F
Nucleic Acids Res. 1984 Jun 25;12(12):4987-95. doi: 10.1093/nar/12.12.4987.
The nusB gene of E. coli has been cloned in plasmid pBR322. Using genetic complementation as an assay for the gene, its location in subclones was analyzed, and the nucleotide sequence of this gene and its flanking regions was determined. The coding region consists of 417 base pairs (bp), which specify a protein of 139 amino acids, and the calculated molecular weight of the nusB protein is 15,702. The nusB protein contains 20 acidic and 21 basic amino acids. A significant promoter sequence was not found to be located in the region upstream from the translational initiation codon. The possibility that the nusB gene consists of an operon is discussed. After the coding region, there is a G-C rich inverted repeat sequence followed by a run of Ts, which could be a transcriptional terminator of the nusB gene.
大肠杆菌的nusB基因已被克隆到质粒pBR322中。以遗传互补作为该基因的检测方法，分析了其在亚克隆中的位置，并测定了该基因及其侧翼区域的核苷酸序列。编码区由417个碱基对（bp）组成，指定了一个由139个氨基酸组成的蛋白质，nusB蛋白的计算分子量为15702。nusB蛋白含有20个酸性氨基酸和21个碱性氨基酸。在翻译起始密码子上游区域未发现明显的启动子序列。讨论了nusB基因由一个操纵子组成的可能性。在编码区之后，有一个富含G-C的反向重复序列，后面跟着一串T，这可能是nusB基因的转录终止子。
