Souvras M, Montagnon B, Fanget B, van Wezel A L, Hazendonk A G
Dev Biol Stand. 1980;46:197-202.
The proposed micro-ELISA assay by means of the double antibody method involves three steps: adsorption of type specific antiserum on micro-wells; simultaneous incubation of antigen and Horse Radish Peroxydase (HRPO) conjugated antiserum; substrate incubation followed by photometric measurement of absorbance at 403 nm. Preliminary results seem generally in good agreement with those obtained by other tests such as gel diffusion and indirect ELISA.
将型特异性抗血清吸附于微孔板上；同时孵育抗原和辣根过氧化物酶（HRPO）偶联抗血清；进行底物孵育，随后在403nm处进行吸光度的光度测量。初步结果似乎总体上与通过其他检测方法（如凝胶扩散和间接酶联免疫吸附测定）所获得的结果高度一致。
