van der Marel P, Hazendonk A G, van Wezel A L
Dev Biol Stand. 1981;47:101-8.
Up to now gel diffusion is widely used for in vitro determination of polio D-antigen. This method has several disadvantages, its relatively low sensitivity being the main one. Generally samples have to be concentrated, which requires fairly large amounts of material and often results in some loss of antigen due to adsorption. Two ELISA methods have been developed for determination of polio D-antigen. These tests have a high sensitivity, approximately 1 DU/ml, and require very small amounts of sample and antisera. Using the indirect method only one HRPO conjugated antiserum is needed for quantitation of type 1, 2 and 3 D-antigen. Comparison of D-antigen values obtained by ELISA and gel diffusion for a number of vaccines revealed a good correlation between the different methods. The indirect ELISA method has also been applied successfully for D-antigen quantitation of A1PO4 adsorbed DT-and DPT-polio vaccines.
到目前为止，凝胶扩散法广泛用于体外测定脊髓灰质炎D抗原。该方法有几个缺点，其中主要缺点是灵敏度相对较低。通常样品必须浓缩，这需要相当大量的材料，并且由于吸附作用常常导致一些抗原损失。已经开发出两种用于测定脊髓灰质炎D抗原的ELISA方法。这些检测方法灵敏度高，约为1 DU/ml，并且需要非常少量的样品和抗血清。使用间接法，定量1型、2型和3型D抗原仅需要一种与辣根过氧化物酶（HRPO）结合的抗血清。通过ELISA和凝胶扩散法对多种疫苗获得的D抗原值进行比较，结果表明不同方法之间具有良好的相关性。间接ELISA法也已成功应用于吸附于磷酸铝（A1PO4）的白喉破伤风联合疫苗（DT）和白百破脊髓灰质炎联合疫苗（DPT）的D抗原定量。
