苯甲基磺酰氟在酿酒酵母完整细胞中分解代谢失活和阻遏研究中的应用。

The use of phenylmethylsulfonyl fluoride in the study of catabolite inactivation and repression in intact cells of Saccharomyces cervisiae.

作者信息

Grossmann M K

出版信息

Arch Microbiol. 1980 Feb;124(2-3):293-5. doi: 10.1007/BF00427741.

Abstract

Catabolite inactivation of fructose-1,6-bisphosphatase, isocitrate lyase, phosphoenolpruvate carboxykinase and malate dehydrogenase in intact cells could be prevented by phenylmethylsulfonyl fluoride added 40 min prior to the addition of glucose. Protein synthesis, fermentative and respiratory activity and catabolite repression were not affected. Elimination of catabolite inactivation by the addition of PMSF revealed that catabolite repression started at different times for different enzyme.

摘要

在完整细胞中，果糖-1,6-二磷酸酶、异柠檬酸裂解酶、磷酸烯醇丙酮酸羧激酶和苹果酸脱氢酶的分解代谢失活可通过在添加葡萄糖前40分钟添加苯甲基磺酰氟来防止。蛋白质合成、发酵和呼吸活性以及分解代谢阻遏均未受影响。添加苯甲基磺酰氟消除分解代谢失活表明，不同酶的分解代谢阻遏在不同时间开始。