Bruscalupi G, Curatola G, Lenaz G, Leoni S, Mangiantini M T, Mazzanti L, Spagnuolo S, Trentalance A
Biochim Biophys Acta. 1980 Apr 10;597(2):263-73. doi: 10.1016/0005-2736(80)90104-2.
The lipid composition and fluidity of plasma membranes have been studied at different stages of liver regeneration (4, 15 and 24 h after surgery). The phospholipid and fatty acid composition is not modified, whereas the cholesterol/phospholipid ratio is lower with respect to control membranes. The modification of the physical properties of the membranes has been studied directly by EPR analysis and indirectly by temperature dependence and cooperativity of some membrane-bound enzymes (Mg2+-ATPase, (Na+ + K+)-ATPase and 5'nucleotidase). Surgical operation or anaesthesia alone causes an early increase in fluidity; such an effect appears to be markedly reduced at a later stage. There seems to be a marked effect of regeneration on plasma membrane fluidity 15 h after partial hepatectomy when several parameters--surface fluidity, cholesterol/phospholipid ratio, and 5'-nucleotidase activity in the presence of concanavalin A -- are modified and indicate an increase in membrane fluidity. It is suggested that this modification of membrane properties could be related to the proliferative process.
在肝脏再生的不同阶段（手术后4小时、15小时和24小时）对质膜的脂质组成和流动性进行了研究。磷脂和脂肪酸组成未发生改变，而相对于对照膜，胆固醇/磷脂比值较低。通过电子顺磁共振分析直接研究了膜物理性质的改变，并通过一些膜结合酶（Mg2 + -ATP酶、（Na + + K +）-ATP酶和5'-核苷酸酶）的温度依赖性和协同性间接进行了研究。单独的外科手术或麻醉会导致流动性早期增加；这种效应在后期似乎会明显降低。在部分肝切除术后15小时，当几个参数——表面流动性、胆固醇/磷脂比值以及伴刀豆球蛋白A存在时的5'-核苷酸酶活性——发生改变并表明膜流动性增加时，再生对质膜流动性似乎有显著影响。有人认为，膜性质的这种改变可能与增殖过程有关。
