Pietrangelo A, Tripodi A, Carulli N, Tomasi A, Ceccarelli D, Ventura E, Masini A
Clinica Medica III, Università di Modena, Italy.
J Bioenerg Biomembr. 1989 Aug;21(4):527-33. doi: 10.1007/BF00762524.
Liver plasma membranes isolated from rats with chronic dietary iron overload showed a large modification of their phospholipid fatty acid composition. Specifically, a significant decrease in polyunsaturated fatty acids and a parallel increase in saturated fatty acids was observed. This pattern was consistent with the in vivo occurrence of lipoperoxidative reactions in the liver plasma membranes. However, neither change in the cholesterol/phospholipid molar ratio nor in the lipid/protein ratio was detected. Direct measurement of the plasma membrane fluidity state by electron spin resonance spectrometry did not reveal any difference between control and iron-treated rats. These findings indicate that chronic dietary iron overload can induce lipid peroxidation of rat liver plasma membranes, but this event does not bring about modification in the physical state of the membrane.
从患有慢性膳食铁过载的大鼠中分离出的肝质膜显示出其磷脂脂肪酸组成有很大改变。具体而言，观察到多不饱和脂肪酸显著减少，饱和脂肪酸相应增加。这种模式与肝质膜中体内脂质过氧化反应的发生一致。然而，未检测到胆固醇/磷脂摩尔比或脂质/蛋白质比的变化。通过电子自旋共振光谱法直接测量质膜流动性状态未发现对照大鼠和铁处理大鼠之间有任何差异。这些发现表明，慢性膳食铁过载可诱导大鼠肝质膜脂质过氧化，但这一事件不会导致膜的物理状态发生改变。
