Ben-Asher E, Strauss B
Biochim Biophys Acta. 1980 Mar 28;607(1):10-22. doi: 10.1016/0005-2787(80)90216-6.
Bacteriophage T7 DNA reacts uniformly with trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene(anti-BPDE). The reaction product retains the native configuration so that only one site sensitive to S1 nuclease is produced for every 70 anti-BPDE adducts. DNA treated with anti-BPDE is retained on benzoylated naphthoylated DEAE-cellulose even after washing with 1.0 M salt solutions. About 100 adducts per T7 molecule are required for adherence which is not due to breaks or single-stranded regions since adherence is not affected by S1 nuclease treatment. The binding of anti-BPDE reacted DNA to benzoylated naphthoylated DEAE-cellulose is cooperative and requires many residues per bound fragment. Treatment of T7 DNA treated with anti-BPDE with restriction endonuclease yields smaller molecules, still containing adducts, which do not adhere. We interpret these results to mean that reaction with BPDE does not involve deformation of the DNA structure and that the adducts lie in a position which they are readily accessible for interaction with aromatic groups on the column resin.
噬菌体T7 DNA与反式-7,8-二羟基-反-9,10-环氧-7,8,9,10-四氢苯并[a]芘（反式-BPDE）均匀反应。反应产物保留天然构象，因此每70个反式-BPDE加合物仅产生一个对S1核酸酶敏感的位点。用反式-BPDE处理过的DNA即使在用1.0M盐溶液洗涤后仍保留在苯甲酰化萘甲酰化DEAE-纤维素上。每个T7分子大约需要100个加合物才能实现粘附，这不是由于断裂或单链区域造成的，因为粘附不受S1核酸酶处理的影响。反式-BPDE反应的DNA与苯甲酰化萘甲酰化DEAE-纤维素的结合是协同的，每个结合片段需要许多残基。用限制性内切酶处理用反式-BPDE处理过的T7 DNA会产生较小的分子，这些分子仍含有加合物，但不发生粘附。我们将这些结果解释为，与BPDE的反应不涉及DNA结构的变形，并且加合物所处的位置使其易于与柱树脂上的芳香族基团相互作用。
