Sherr C J, Fedele L A, Oskarsson M, Maizel J, Vande Woude G
J Virol. 1980 Apr;34(1):200-12. doi: 10.1128/JVI.34.1.200-212.1980.
Extrachromosomal DNA obtained from mink cells acutely infected with the Snyder-Theilen (ST) strain of feline sarcoma virus (feline leukemia virus) [FeSV(FeLV)] was fractionated electrophoretically, and samples enriched for FeLV and FeSV linear intermediates were digested with EcoRI and cloned in lambda phage. Hybrid phages were isolated containing either FeSV or FeLV DNA "inserts" and were characterized by restriction enzyme analysis, R-looping with purified 26 to 32S viral RNA, and heteroduplex formation. The recombinant phages (designated lambda FeSV and lambda FeLV) contain all of the genetic information represented in FeSV and FeLV RNA genomes but lack one extended terminally redundant sequence of 750 bases which appears once at each end of parental linear DNA intermediates. Restriction enzyme and heteroduplex analyses confirmed that sequences unique to FeSV (src sequences) are located at the center of the FeSV genome and are approximately 1.5 kilobase pairs in length. With respect to the 5'-3' orientation of genes in viral RNA, the order of genes in the FeSV genome is 5'-gag-src-env-c region-3'; only 0.9 kilobase pairs of gag and 0.6 kilobase pairs of env-derived FeLV sequences are represented in ST FeSV. Heteroduplex analyses between lambda FeSV or lambda FeLV DNA and Moloney murine sarcoma virus DNA (strain m1) were performed under conditions of reduced stringency to demonstrate limited regions of base pair homology. Two such regions were identified: the first occurs at the extreme 5' end of the leukemia and both sarcoma viral genomes, whereas the second corresponds to a 5' segment of leukemia virus "env" sequences conserved in both sarcoma viruses. The latter sequences are localized at the 3' end of FeSV src and at the 5' end of murine sarcoma virus src and could possibly correspond to regions of helper virus genomes that are required for retroviral transforming functions.
从急性感染 Snyder-Theilen（ST）株猫肉瘤病毒（猫白血病病毒）[FeSV(FeLV)]的水貂细胞中获得的染色体外 DNA 进行了电泳分级分离，富集了 FeLV 和 FeSV 线性中间体的样品用 EcoRI 消化并克隆到λ噬菌体中。分离出含有 FeSV 或 FeLV DNA“插入片段”的杂交噬菌体，并通过限制性内切酶分析、与纯化的 26 至 32S 病毒 RNA 进行 R 环分析以及异源双链体形成进行表征。重组噬菌体（命名为λFeSV 和λFeLV）包含 FeSV 和 FeLV RNA 基因组中所代表的所有遗传信息，但缺少一个 750 个碱基的延伸末端冗余序列，该序列在亲本线性 DNA 中间体的两端各出现一次。限制性内切酶和异源双链体分析证实，FeSV 特有的序列（src 序列）位于 FeSV 基因组的中心，长度约为 1.5 千碱基对。就病毒 RNA 中基因的 5'-3'方向而言，FeSV 基因组中基因的顺序是 5'-gag-src-env-c 区域-3'；在 ST FeSV 中仅代表 0.9 千碱基对的 gag 和 0.6 千碱基对的 env 衍生的 FeLV 序列。在降低严谨性的条件下对λFeSV 或λFeLV DNA 与莫洛尼鼠肉瘤病毒 DNA（菌株 m1）之间进行异源双链体分析，以证明有限的碱基对同源区域。鉴定出两个这样的区域：第一个出现在白血病和两种肉瘤病毒基因组的极端 5'端，而第二个对应于两种肉瘤病毒中保守的白血病病毒“env”序列的 5'段。后一个序列位于 FeSV src 的 3'端和鼠肉瘤病毒 src 的 5'端，可能对应于逆转录病毒转化功能所需的辅助病毒基因组区域。
