Beaman B L, Burtis K C, Doi R H, Yeggy J P, Stahly D P
Can J Microbiol. 1980 Apr;26(4):420-6. doi: 10.1139/m80-070.
An electron microscopic analysis of Bacillus subtilis cells revealed that netropsin blocked sporulation ultrastructurally at stages 0-I. These observations are consistent with previous results which indicated that cells were not committed to sporulation in the presence of the drug. Further, the addition of netropsin up to stage III of sporulation prevented the normal sharp increase in dihydrodipicolinate synthase activity which results in dipicolinic acid accumulation of stage IV. The addition of netropsin after stage III had much less effect on the synthesis of dihydrodipicolinate synthase and on sporulation. Thus, morphological events in sporulation are blocked early whereas a sporulation-associated enzyme may be affected at later stages. These data indicate than netropsin affects the expression of sporulation-associated genes in a differential manner.
对枯草芽孢杆菌细胞的电子显微镜分析显示，纺锤菌素在超微结构上于孢子形成的0-I阶段阻断了孢子形成。这些观察结果与先前的结果一致，先前结果表明在药物存在下细胞不会进入孢子形成阶段。此外，在孢子形成的III阶段之前添加纺锤菌素可阻止二氢吡啶二羧酸合酶活性正常急剧增加，而这种增加会导致IV阶段吡啶二羧酸的积累。在III阶段之后添加纺锤菌素对二氢吡啶二羧酸合酶的合成和孢子形成的影响要小得多。因此，孢子形成中的形态学事件在早期被阻断，而与孢子形成相关的酶可能在后期受到影响。这些数据表明，纺锤菌素以不同的方式影响与孢子形成相关基因的表达。
