Hallewell R A, Emtage S
Gene. 1980 Apr;9(1-2):27-47. doi: 10.1016/0378-1119(80)90165-1.
Derivatives of plasmid pBR322 that are suitable for high-level expression of foreign genes have been constructed. The vectors contain the Escherichia coli tryptophan promoter, the trpE gene, and about 15% of the trpD gene. To obtain expression, foreign genes are fused to the trpD gene fragment. After induction of the trp operon with 3 beta-indolylacrylic acid, trp gene products increase at least 50-fold, to account for 55% of the newly synthesised protein and 30% of total protein in the cell.
已构建出适用于外源基因高水平表达的质粒pBR322衍生物。这些载体包含大肠杆菌色氨酸启动子、trpE基因和约15%的trpD基因。为实现表达，将外源基因与trpD基因片段融合。用3-β-吲哚丙烯酸诱导trp操纵子后，trp基因产物增加至少50倍，占细胞中新合成蛋白质的55%和总蛋白质的30%。
