Kurokawa T, Seno M, Sasada R, Ono Y, Onda H, Igarashi K, Kikuchi M, Sugino Y, Honjo T
Nucleic Acids Res. 1983 May 25;11(10):3077-85. doi: 10.1093/nar/11.10.3077.
Using the cDNA of human epsilon chain, three expression plasmids that code directly the constant portion of the epsilon chain (C epsilon 1-C epsilon 4, C epsilon 2-C epsilon 4 and C epsilon 3-C epsilon 4 domains) were constructed. These epsilon chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human epsilon chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.
利用人ε链的互补脱氧核糖核酸（cDNA）构建了三种直接编码ε链恒定区（Cε1 - Cε4、Cε2 - Cε4和Cε3 - Cε4结构域）的表达质粒。这些ε链肽在色氨酸启动子 - 操纵子的控制下于大肠杆菌中合成。细菌产生的肽具有人ε链的抗原性，其分子量与根据构建质粒的氨基酸序列计算出的值相等。
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