Norrild B, Shore S L, Cromeans T L, Nahmias A J
Infect Immun. 1980 Apr;28(1):38-44. doi: 10.1128/iai.28.1.38-44.1980.
Tissue culture cells infected with herpes simplex virus type 1 synthesize three major glycoprotein antigens (Ag-11, Ag-8, and Ag-6), which have been characterized by crossed immunoelectrophoresis. The three viral antigens have been identified as a mixture of gA and gB (Ag-11), gD (Ag-8), and gC (Ag-6). Recent findings have shown that antibodies directed to each of the three antigens individually are able to mediate antibody-dependent cell-mediated cytotoxicity when tissue culture cells late in the infectious cycle (18 h postinfection) are used. In this work, antibody-dependent cell-mediated cytotoxicity was applied to study the time postinfection at which the individual viral antigens first made their appearance at the cell surface. All three viral antigens (Ag-11, Ag-8, and Ag-6) could be demonstrated as newly synthesized from 3 to 4 h postinfection, and the quantities of the antigens at the surfaces of the infected cells increased with time postinfection. The use of cycloheximide and ultraviolet-inactivated virus demonstrated that input virus could be detected by antibody-dependent cell-mediated cytotoxicity during the first 2 h postinfection, but the cytotoxicity caused by input virus remained constant with time postinfection. In conclusion, these observations demonstrate the participation of individual herpes simplex virus surface antigens in antibody-dependent cell-mediated cytotoxicity attack on cells early in infection.
感染单纯疱疹病毒1型的组织培养细胞可合成三种主要糖蛋白抗原（Ag-11、Ag-8和Ag-6），这些抗原已通过交叉免疫电泳进行了表征。这三种病毒抗原已被鉴定为gA和gB（Ag-11）、gD（Ag-8）和gC（Ag-6）的混合物。最近的研究结果表明，当使用感染周期后期（感染后18小时）的组织培养细胞时，针对这三种抗原中每一种的抗体都能够介导抗体依赖性细胞介导的细胞毒性。在这项工作中，抗体依赖性细胞介导的细胞毒性被用于研究感染后个体病毒抗原首次出现在细胞表面的时间。所有三种病毒抗原（Ag-11、Ag-8和Ag-6）在感染后3至4小时都可被证明是新合成的，并且感染细胞表面的抗原量随感染后时间增加。使用环己酰亚胺和紫外线灭活病毒表明，在感染后最初2小时内，输入病毒可通过抗体依赖性细胞介导的细胞毒性检测到，但输入病毒引起的细胞毒性在感染后随时间保持恒定。总之，这些观察结果证明了单纯疱疹病毒个体表面抗原在感染早期对细胞的抗体依赖性细胞介导的细胞毒性攻击中的参与。
