Vestergaard B F, Bjerrum O J, Norrild B, Grauballe P C
J Virol. 1977 Oct;24(1):82-90. doi: 10.1128/JVI.24.1.82-90.1977.
The nonionic detergent Triton X-100 was capable of solubilizing 90% of the protein content in herpes simplex virus (HSV)-infected rabbit cornea cells. The solubilized HSV antigens formed well-characterized precipitates by crossed immunoelectrophoresis in Triton X-100-containing agarose gel, allowing both identification and relative quantitation. Water-soluble and detergent-requiring HSV antigens were identified by different solubilization procedures in buffer with and without detergent. Five glycoprotein antigens were solubilized only in the presence of detergent, indicating their membrane-bound state. One non-glycosylated antigen was present in both a water-soluble and a membrane-bound form. Based upon the crossed immunoelectrophoretic precipitating patterns of Triton X-100-solubilized HSV antigens, it has been estimated that infected cells yield an amount of virus-specific protein equivalent to 2,000 enveloped virions per cell. Rabbits inoculated intracutaneously with Triton X-100-solubilized HSV antigens developed neutralizing antibodies against HSV almost as effectively as rabbits with an active HSV infection. Precipitins against individual HSV antigens in sera from rabbits infected with HSV and immunized with the Triton X-100-solubilized HSV antigens were assayed by the crossed immunoelectrophoretic technique. Sera from infected rabbits reacted more strongly and with a higher number of HSV antigens than sera from immunized rabbits.
非离子去污剂Triton X-100能够溶解单纯疱疹病毒（HSV）感染的兔角膜细胞中90%的蛋白质。在含Triton X-100的琼脂糖凝胶中，通过交叉免疫电泳，溶解的HSV抗原形成了特征明确的沉淀，从而实现了鉴定和相对定量。通过在含和不含去污剂的缓冲液中采用不同的溶解程序，鉴定了水溶性和需要去污剂的HSV抗原。五种糖蛋白抗原仅在有去污剂存在时才能溶解，表明它们处于膜结合状态。一种非糖基化抗原以水溶性和膜结合两种形式存在。根据Triton X-100溶解的HSV抗原的交叉免疫电泳沉淀模式，估计受感染细胞产生的病毒特异性蛋白量相当于每个细胞2000个包膜病毒粒子。皮内接种Triton X-100溶解的HSV抗原的兔子产生抗HSV的中和抗体的效果几乎与患有活动性HSV感染的兔子一样有效。通过交叉免疫电泳技术检测感染HSV并用Triton X-100溶解的HSV抗原免疫的兔子血清中针对单个HSV抗原的沉淀素。与免疫兔子的血清相比，感染兔子的血清反应更强，针对的HSV抗原数量更多。
