Middendorf W F, Russell J A
J Appl Physiol Respir Environ Exerc Physiol. 1980 Jun;48(6):947-56. doi: 10.1152/jappl.1980.48.6.947.
The distribution of receptors and the innervation of intrapulmonary and extrapulmonary baboon airways were examined using tissue bath techniques. Acetylcholine and histamine (via H1-receptors) elicited contractions of all airways. Norepinephrine had both an excitatory (via alpha-receptors) and inhibitory (via beta-receptors) effect in trachealis strips, but in intrapulmonary airways, only inhibitions were observed. Transmural electrical stimulation of resting airways produced sustained contractions that were blocked by either 5 x 10(-6) M tetrodotoxin or 10(-6) M atropine. Electrical stimulation also caused relaxations of histamine-induced contractions in airways pretreated with 10(-6) M atropine and 10(-6) M phentolamine. Relaxations were blocked by 10 x 10(-6) M tetrodotoxin but were unaffected by either 10(-6) M propranolol or 10(-5) M guanethidine. The response of airways to exogenous ATP depended on the degree of active tone. ATP caused contractions in resting airways and airways with moderate tone. In contrast, ATP relaxed airways with high active tone. We conclude that a nonadrenergic system constitute the primary inhibitory innervation of baboon airways, but whether ATP might act as the neurotransmitter could not be determined.
运用组织浴技术研究了狒狒肺内和肺外气道的受体分布及神经支配情况。乙酰胆碱和组胺（通过H1受体）可引起所有气道收缩。去甲肾上腺素对气管条有兴奋作用（通过α受体）和抑制作用（通过β受体），但在肺内气道中，仅观察到抑制作用。对静息气道进行跨壁电刺激可产生持续收缩，该收缩可被5×10⁻⁶ M的河豚毒素或10⁻⁶ M的阿托品阻断。电刺激还可使经10⁻⁶ M阿托品和10⁻⁶ M酚妥拉明预处理的气道中组胺诱导的收缩松弛。松弛作用可被10×10⁻⁶ M的河豚毒素阻断，但不受10⁻⁶ M普萘洛尔或10⁻⁵ M胍乙啶的影响。气道对外源性ATP的反应取决于主动张力的程度。ATP可使静息气道和具有适度张力的气道收缩。相反，ATP可使具有高主动张力的气道松弛。我们得出结论，非肾上腺素能系统构成狒狒气道的主要抑制性神经支配，但无法确定ATP是否可能作为神经递质起作用。
