Sabara M, Babiuk L A
J Virol. 1984 Aug;51(2):489-96. doi: 10.1128/JVI.51.2.489-496.1984.
Preliminary characterization of naturally occurring temperature-sensitive bovine rotavirus mutants, generated via gene reassortment, indicated that the product of gene segment 10 can influence virus adsorption to MA-104 cells. Cell-free translation of this gene segment in a rabbit reticulocyte system was then utilized to identify a polypeptide with a molecular weight of ca. 21,500 as the primary translation product. This identification provides a basis for further studies of rotavirus assembly and cell interactions which may, in turn, aid in elucidating the replicative strategy of the virus.
对通过基因重配产生的天然存在的温度敏感型牛轮状病毒突变体的初步表征表明，基因片段10的产物可影响病毒对MA-104细胞的吸附。然后利用兔网织红细胞系统对该基因片段进行无细胞翻译，以鉴定出一种分子量约为21,500的多肽作为主要翻译产物。这一鉴定为进一步研究轮状病毒组装和细胞相互作用提供了基础，而这些研究反过来可能有助于阐明该病毒的复制策略。
